Development and chromosomal localization of genome-specific markers by polymerase chain reaction in Brassica

C F Quiros; J Hu; P This; A M Chevre; M Delseny

doi:10.1007/BF00226801

Development and chromosomal localization of genome-specific markers by polymerase chain reaction in Brassica

Theor Appl Genet. 1991 Oct;82(5):627-32. doi: 10.1007/BF00226801.

Authors

C F Quiros¹, J Hu, P This, A M Chevre, M Delseny

Affiliation

¹ Department of Vegetable Crops, University of California, 95616, Davis, CA, USA.

PMID: 24213344
DOI: 10.1007/BF00226801

Abstract

This paper reports the application of the RAPD (random amplification of polymorphic DNA sequence) markers in Brassica genetics. Forty-seven arbitrary decamer oligonucletides were used as primers to amplify genomic DNA by polymerase chain reaction. Some of the amplified products were genome specific and could be found in both diploid and derived amphidiploid species. Of a total of 65 such markers, 16 were A genome, 37 B genome, and 12 C genome specific. Of the 37 B-genome-specific markers, 11 were mapped on four independent chromosomes of B. nigra with the aid of existing B. napus-nigra disomic alien addition lines.