Objective: To construct and characterize EGFP reporter gene labeled Sindbis virus (SINV).
Methods: The reporter gene EGFP was inserted into the genome of infectious clone pBR-XJ160 by using multi-fusion long fragment PCR method. Then apply reverse genetic manipulation technique to rescue and obtain EGFP labeled SINV.
Results: We successively obtained labeled SINV, which has good fluorescent expression characteristics and genetic stability.
Conclusion: The labeled virus can be seen in living cells and living body, and this serves as a good tool for cell and tissue tropism and biological function study of viruses. This study laid a foundation for further studying the cell tropism, biological functions and infection mechanism of SINV.