Isolation and characterization of numerous novel phages targeting diverse strains of the ubiquitous and opportunistic pathogen Achromobacter xylosoxidans

PLoS One. 2014 Jan 22;9(1):e86935. doi: 10.1371/journal.pone.0086935. eCollection 2014.

Abstract

The clinical relevance of nosocomially acquired infections caused by multi-resistant Achromobacter strains is rapidly increasing. Here, a diverse set of 61 Achromobacter xylosoxidans strains was characterized by MultiLocus Sequence Typing and Phenotype MicroArray technology. The strains were further analyzed in regard to their susceptibility to 35 antibiotics and to 34 different and newly isolated bacteriophages from the environment. A large proportion of strains were resistant against numerous antibiotics such as cephalosporines, aminoglycosides and quinolones, whereas piperacillin-tazobactam, ticarcillin, mezlocillin and imipenem were still inhibitory. We also present the first expanded study on bacteriophages of the genus Achromobacter that has been so far a blank slate with respect to phage research. The phages were isolated mainly from several waste water treatment plants in Germany. Morphological analysis of all of these phages by electron microscopy revealed a broad diversity with different members of the order Caudovirales, including the families Siphoviridae, Myoviridae, and Podoviridae. A broad spectrum of different host ranges could be determined for several phages that lysed up to 24 different and in part highly antibiotic resistant strains. Molecular characterisation by DNA restriction analysis revealed that all phages contain linear double-stranded DNA. Their restriction patterns display distinct differences underlining their broad diversity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Achromobacter denitrificans / drug effects*
  • Achromobacter denitrificans / genetics*
  • Achromobacter denitrificans / virology*
  • Anti-Bacterial Agents / pharmacology*
  • Blotting, Southern
  • Caudovirales / ultrastructure*
  • Drug Resistance, Bacterial / genetics*
  • Germany
  • Microarray Analysis
  • Microscopy, Electron
  • Multilocus Sequence Typing
  • Phylogeny
  • Polymorphism, Restriction Fragment Length
  • Species Specificity

Substances

  • Anti-Bacterial Agents

Grants and funding

This work was supported by Leibniz Institute DSMZ - German Collection of Microorganisms and Cell Cultures funded this research through the employment of Johannes Wittmann, Christine Rohde and Johannes Sikorski, who contributed to all aspects of the study. There are no external funders who could have had a role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.