In vivo delivery of lenti-Cre or adeno-Cre into mice using intranasal instillation

Cold Spring Harb Protoc. 2014 Mar 1;2014(3):307-9. doi: 10.1101/pdb.prot073445.

Abstract

Lung cancer remains the leading cause of cancer deaths among both men and women, with a lower rate of survival than both breast and prostate cancer. Development of the Cre/lox system and improved mouse models have allowed researchers to gain a better understanding of human disease, including lung cancer. Through the viral delivery of Cre, gene function in adult mice can be precisely studied at a specific developmental stage or in a specific cell/tissue type of choice. This protocol describes how to produce adenovirus-Cre precipitate. Using this adeno-Cre (or lentivirus-Cre), Cre can be expressed in mouse lungs. The virus is delivered by intranasal instillation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenoviridae / genetics*
  • Administration, Intranasal
  • Animals
  • Genetic Vectors / administration & dosage*
  • Integrases / genetics
  • Lentivirus / genetics*
  • Mice

Substances

  • Cre recombinase
  • Integrases