Coomassie blue staining

Methods Enzymol. 2014:541:161-7. doi: 10.1016/B978-0-12-420119-4.00013-6.

¹ Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, Baltimore, MD, USA; Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
² Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, Baltimore, MD, USA; Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD, USA. Electronic address: ragreen@jhmi.edu.

Abstract

This protocol describes Coomassie brilliant blue staining, one of the most common methods of detecting proteins in polyacrylamide gels (PAGE).

Keywords: Coomassie blue staining; G-250; Kimwipes or paper towels; Polyacrylamide gels (PAGE); R-250; Silver staining; Whatman 1 filter paper.