A rapid, site-selective and efficient route to the dual modification of DARPins

Paul Moody; Vijay Chudasama; Ramiz I Nathani; Antoine Maruani; Stephen Martin; Mark E B Smith; Stephen Caddick

doi:10.1039/c4cc00053f

A rapid, site-selective and efficient route to the dual modification of DARPins

Chem Commun (Camb). 2014 May 18;50(38):4898-900. doi: 10.1039/c4cc00053f.

Authors

Paul Moody¹, Vijay Chudasama, Ramiz I Nathani, Antoine Maruani, Stephen Martin, Mark E B Smith, Stephen Caddick

Affiliation

¹ Department of Chemistry, University College London, 20 Gordon Street, London, WC1H 0AJ, UK. vpenterprise@ucl.ac.uk.

Abstract

Designed ankyrin repeat proteins (DARPins) are valuable tools in both biochemistry and medicine. Herein we describe a rapid, simple method for the dual modification of DARPins by introduction of cysteine mutations at specific positions that results in a vast difference in their thiol nucleophilicity, allowing for clean sequential modification.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Ankyrins / chemistry
Ankyrins / genetics
Ankyrins / metabolism*
Circular Dichroism
Cysteine / metabolism
Humans
Mutagenesis, Site-Directed
Protein Binding
Protein Engineering
Receptor, ErbB-2 / chemistry
Receptor, ErbB-2 / metabolism
Recombinant Proteins / biosynthesis
Recombinant Proteins / chemistry
Recombinant Proteins / genetics

Substances

Ankyrins
Recombinant Proteins
Receptor, ErbB-2
Cysteine

Abstract

Publication types

MeSH terms

Substances

Grants and funding