Characterization of a novel Pseudomonas aeruginosa bacteriophage, KPP25, of the family Podoviridae

Reina Miyata; Kotoe Yamaguchi; Jumpei Uchiyama; Ryu Shigehisa; Iyo Takemura-Uchiyama; Shin-ichiro Kato; Takako Ujihara; Yoshihiko Sakaguchi; Masanori Daibata; Shigenobu Matsuzaki

doi:10.1016/j.virusres.2014.04.019

Characterization of a novel Pseudomonas aeruginosa bacteriophage, KPP25, of the family Podoviridae

Virus Res. 2014 Aug 30:189:43-6. doi: 10.1016/j.virusres.2014.04.019. Epub 2014 May 5.

Affiliations

¹ Department of Microbiology and Infection, Faculty of Medicine, Kochi University, Kochi, Japan; Center for Innovative and Translational Medicine, Faculty of Medicine, Kochi University, Kochi, Japan.
² Department of Microbiology and Infection, Faculty of Medicine, Kochi University, Kochi, Japan; Center for Innovative and Translational Medicine, Faculty of Medicine, Kochi University, Kochi, Japan. Electronic address: jumpeiu@kochi-u.ac.jp.
³ Department of Microbiology and Infection, Faculty of Medicine, Kochi University, Kochi, Japan.
⁴ Research Institute of Molecular Genetics, Kochi University, Kochi, Japan.
⁵ Science Research Center, Kochi University, Kochi, Japan.
⁶ Interdisciplinary Research Organization, University of Miyazaki, Miyazaki, Japan.

PMID: 24801109
DOI: 10.1016/j.virusres.2014.04.019

Abstract

Pseudomonas aeruginosa phages belonging to the family Podoviridae are one of the well-characterized phage groups. In this study, a novel P. aeruginosa phage, KPP25, was isolated and characterized. Phage KPP25's morphology was indicative of the family Podoviridae; however, analyses of the whole genome and the virion proteins suggested that it did not belong to any of the known podophage genera. Based on these analyses, phage KPP25 appears to be a novel podophage infecting P. aeruginosa.

Keywords: Bacteriophage; Podoviridae; Pseudomonas aeruginosa.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA, Viral / chemistry*
DNA, Viral / genetics*
Gene Order
Genome, Viral*
Humans
Microscopy, Electron, Transmission
Molecular Sequence Data
Podoviridae / genetics*
Podoviridae / isolation & purification
Podoviridae / ultrastructure
Pseudomonas Phages / genetics*
Pseudomonas Phages / isolation & purification
Pseudomonas Phages / ultrastructure
Pseudomonas aeruginosa / virology*
Sequence Analysis, DNA

Substances

DNA, Viral

Associated data

GENBANK/AB910393
RefSeq/NC_024123