Comparative evaluation of two chromogenic tests for rapid detection of carbapenemase in Enterobacteriaceae and in Pseudomonas aeruginosa isolates

Te-Din Huang; Catherine Berhin; Pierre Bogaerts; Youri Glupczynski

doi:10.1128/JCM.00643-14

Comparative evaluation of two chromogenic tests for rapid detection of carbapenemase in Enterobacteriaceae and in Pseudomonas aeruginosa isolates

J Clin Microbiol. 2014 Aug;52(8):3060-3. doi: 10.1128/JCM.00643-14. Epub 2014 May 21.

Authors

Te-Din Huang¹, Catherine Berhin², Pierre Bogaerts², Youri Glupczynski²

Affiliations

¹ National Reference Laboratory for Monitoring of Antimicrobial Resistance in Gram-Negative Bacteria, CHU Dinant-Godinne, UCL Namur, Yvoir, Belgium te-din.huang@uclouvain.be.
² National Reference Laboratory for Monitoring of Antimicrobial Resistance in Gram-Negative Bacteria, CHU Dinant-Godinne, UCL Namur, Yvoir, Belgium.

Abstract

We compared the performance of the Carba NP test and the Rosco Rapid CARB screen kit for detecting carbapenemase-producing Enterobacteriaceae and Pseudomonas aeruginosa. Both tests are rapid and highly sensitive; however, the Carba NP test showed superior specificity, and several uninterpretable results were observed with the Rapid CARB screen.

Publication types

Comparative Study
Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Proteins / analysis*
Chromogenic Compounds / analysis*
Enterobacteriaceae / enzymology*
Humans
Microbial Sensitivity Tests / methods
Pseudomonas aeruginosa / enzymology*
Sensitivity and Specificity
Time Factors
beta-Lactamases / analysis*

Substances

Bacterial Proteins
Chromogenic Compounds
beta-Lactamases
carbapenemase