A simple and rapid method for visualization of male meiotic chromosomes in Arabidopsis thaliana

Biosci Biotechnol Biochem. 2014;78(6):1026-8. doi: 10.1080/09168451.2014.905191. Epub 2014 Jun 13.

Abstract

Meiotic chromosomes are of basic interest to the geneticist and cell biologist who study their behavior. A rapid and highly repeatable method for visualization of meiotic chromosomes is useful. Here we describe a fast staining protocol for Arabidopsis male meiotic chromosomes. Meiocytes were squashed into a labeling buffer, the chromosome morphology could be analyzed using fluorescence without any additional treatment.

Keywords: Arabidopsis; chromosomes; cytogenetics; meiosis; microscopy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / cytology*
  • Chromosomes, Plant / metabolism*
  • Meiosis*
  • Microscopy, Fluorescence
  • Molecular Imaging / methods*
  • Staining and Labeling / methods*
  • Time Factors