Abstract
Fos and Jun co-operatively repress the fos promoter. Removal of all putative Fos/Jun binding sites from the fos promoter neither obliterates the repression by Fos/Jun in transient cotransfection experiments in NIH3T3 cells nor the turn-off kinetics of serum-induced fos expression in stably transfected NIH3T3 cells. The dyad symmetry element (DSE) suffices to subject a promoter to this type of repression. However, one of the putative Fos/Jun binding sites (-292 to -299 and thus located immediately adjacent to the DSE), determines the very low level of basal expression.
MeSH terms
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Animals
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Base Sequence
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Cell Line
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Chloramphenicol O-Acetyltransferase / genetics
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Cloning, Molecular
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DNA Mutational Analysis
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DNA-Binding Proteins / physiology*
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Enhancer Elements, Genetic
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Gene Expression Regulation / physiology
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Promoter Regions, Genetic
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Proto-Oncogene Proteins / genetics
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Proto-Oncogene Proteins / physiology*
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Proto-Oncogene Proteins c-fos
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Proto-Oncogene Proteins c-jun
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Repressor Proteins / genetics
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Repressor Proteins / physiology*
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Transcription Factors / physiology*
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Transcription, Genetic
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Transfection
Substances
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DNA-Binding Proteins
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Proto-Oncogene Proteins
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Proto-Oncogene Proteins c-fos
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Proto-Oncogene Proteins c-jun
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Repressor Proteins
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Transcription Factors
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Chloramphenicol O-Acetyltransferase