Viral protein R of human immunodeficiency virus types 1 and 2 is dispensable for replication and cytopathogenicity in lymphoid cells

J Virol. 1989 Jul;63(7):3205-8. doi: 10.1128/JVI.63.7.3205-3208.1989.

Abstract

Viral protein R (VPR) is conserved in human immunodeficiency virus types 1 and 2 (HIV-1 and HIV-2). To assess its function, we have constructed mutations within the vpr coding regions of HIV-1 and HIV-2 predicted to express truncated VPR products. Infectious virus was produced by each proviral clone and showed similar replication kinetics and cytopathogenicity when compared with the corresponding parental proviral clone.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Blotting, Southern
  • Cell Line
  • DNA, Viral / genetics
  • DNA, Viral / isolation & purification
  • Gene Products, vpr
  • HIV-1 / genetics
  • HIV-1 / pathogenicity
  • HIV-1 / physiology*
  • HIV-2 / genetics
  • HIV-2 / pathogenicity
  • HIV-2 / physiology*
  • Humans
  • Kinetics
  • Lymphocytes / microbiology*
  • Mutation
  • Restriction Mapping
  • Retroviridae Proteins / genetics
  • Retroviridae Proteins / physiology*
  • Virus Replication*
  • vpr Gene Products, Human Immunodeficiency Virus

Substances

  • DNA, Viral
  • Gene Products, vpr
  • Retroviridae Proteins
  • vpr Gene Products, Human Immunodeficiency Virus