An improved procedure for identifying and quantitating protein phosphatases in mammalian tissues

FEBS Lett. 1989 Jul 3;250(2):596-600. doi: 10.1016/0014-5793(89)80803-8.

Abstract

The type 2A protein phosphatases in mammalian tissue extracts are inhibited completely and specifically by 1-2 nM okadaic acid. In contrast, type 1 protein phosphatases are hardly affected at these concentrations, complete inhibition requiring 1 microM okadaic acid. These observations have been exploited to develop an improved procedure for the identification and quantitation of type 1, type 2A and type 2C protein phosphatases in tissue extracts.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adrenal Medulla / enzymology
  • Animals
  • Brain / enzymology
  • Cattle
  • Ethers, Cyclic
  • Indicators and Reagents
  • Liver / enzymology
  • Muscles / enzymology
  • Okadaic Acid
  • Phosphoprotein Phosphatases / analysis*
  • Phosphorylation
  • Rabbits
  • Rats
  • Tissue Distribution

Substances

  • Ethers, Cyclic
  • Indicators and Reagents
  • Okadaic Acid
  • Phosphoprotein Phosphatases