Genotyping of CCR5 gene, CCR2b and SDF1 variants related to HIV-1 infection in Gabonese subjects

Intervirology. 2015;58(1):22-6. doi: 10.1159/000369016. Epub 2015 Jan 10.

Abstract

Objective: Given the magnitude of the HIV epidemic infection, many viral and human factors were analyzed, and the most decisive was the variant CCR5-Δ32. The presence of a low HIV prevalence (1.8%) in Gabon in the 1990s, compared to neighboring countries, represents a paradox that led us to search for viral and human genetic variants in this country. In this study, only variants of coreceptors and chemokines were investigated.

Methods: Variants of the coding region of the CCR5 gene were analyzed by denaturing gradient gel electrophoresis, and then variants of SDF1 and CCR2b were determined by polymerase chain reaction-restriction fragment length polymorphism.

Results: Four rare variants of the CCR5 coreceptor were found, while CCR5-Δ32 and CCR5m303 variants were not found. No association with CCR2b-V64I (17%) and SDF1-3'A (2%) variants was determined in relation to HIV-1 infection in Gabonese patients.

Conclusion: The paradox of HIV seroprevalence in Gabon, which ended in the 2000s, was not caused by human genetic variants but rather by environmental factors.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chemokine CXCL12 / genetics*
  • Denaturing Gradient Gel Electrophoresis
  • Environment
  • Gabon / epidemiology
  • Genetic Variation*
  • Genotype
  • HIV Infections / epidemiology*
  • HIV Infections / genetics
  • HIV Seroprevalence
  • HIV-1* / immunology
  • Humans
  • Open Reading Frames
  • Polymerase Chain Reaction
  • Polymorphism, Restriction Fragment Length
  • Prevalence
  • Receptors, CCR2 / genetics*
  • Receptors, CCR5 / genetics*

Substances

  • CCR2 protein, human
  • CCR5 protein, human
  • CXCL12 protein, human
  • Chemokine CXCL12
  • Receptors, CCR2
  • Receptors, CCR5