Abstract
The influence of FGF2 on the efficiency of cardiomyocytes differentiation of mesenchymal stem cells (MSC) of bone marrow induced by 5-azacetidine (5-aza) was studied. The effect of FGF2 developing by the 14th day after the combined action of a differentiating agent and growth factor was manifested in an increase in Mef2A, Mef2D and gene transcription and a rise of ionized Ca2+ concentration in cytoplasm keeping cell viability and proliferation activity. In the presence of FGF2 this approach provided cardiomyogenesis and the increase in the formation of early precursors of cardiomyocytes.
MeSH terms
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Animals
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Azacitidine / pharmacology
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Calcium / metabolism
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Cell Differentiation*
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Cell Proliferation
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Cells, Cultured
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Fibroblast Growth Factor 2 / pharmacology*
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MEF2 Transcription Factors / genetics
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MEF2 Transcription Factors / metabolism
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Mesenchymal Stem Cells / cytology*
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Mesenchymal Stem Cells / drug effects
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Mesenchymal Stem Cells / metabolism
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Myocytes, Cardiac / cytology*
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Myocytes, Cardiac / drug effects
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Myocytes, Cardiac / metabolism
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Rats
Substances
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MEF2 Transcription Factors
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MEF2A protein, rat
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MEF2D protein, rat
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RNA, Messenger
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Fibroblast Growth Factor 2
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Azacitidine
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Calcium