Optimization of extraction efficiency by shear emulsifying assisted enzymatic hydrolysis and functional properties of dietary fiber from deoiled cumin (Cuminum cyminum L.)

Abstract

This study evaluated the optimal conditions for extracting dietary fiber (DF) from deoiled cumin by shear emulsifying assisted enzymatic hydrolysis (SEAEH) using the response surface methodology. Fat adsorption capacity (FAC), glucose adsorption capacity (GAC), and bile acid retardation index (BRI) were measured to evaluate the functional properties of the extracted DF. The results revealed that the optimal extraction conditions included an enzyme to substrate ratio of 4.5%, a reaction temperature of 57 °C, a pH value of 7.7, and a reaction time of 155 min. Under these conditions, DF extraction efficiency and total dietary fiber content were 95.12% and 84.18%, respectively. The major components of deoiled cumin DF were hemicellulose (37.25%) and cellulose (33.40%). FAC and GAC increased with decreasing DF particle size (51-100 μm), but decreased with DF particle sizes <26 μm; BRI increased with decreasing DF particle size. The results revealed that SEAEH is an effective method for extracting DF. DF with particle size 26-51 μm had improved functional properties.

Keywords: Cholesterol (PubChem CID: 5997); Deoiled cumin; Dietary fiber; Disodium hydrogen phosphate dodecahydrate (PubChem CID: 61456); Functional properties; Glucose (PubChem CID: 79025); Hydrochloric acid (PubChem CID: 313); Lignin (PubChem CID: 73555271); Methanol (PubChem CID: 887); Phosphoric acid (PubChem CID: 1004); Response surface methodology; Shear emulsifying assisted enzymatic hydrolysis; Sodium dihydrogen phosphate dehydrate (PubChem CID: 23673460); Sodium hydroxide (PubChem CID: 14798); Taurocholic acid sodium salt hydrate (PubChem CID: 23687511).