Dynamic nano-interfaces enable harvesting of functional 3D-engineered tissues

Akihiro Nishiguchi; Michiya Matsusaki; Shigeru Miyagawa; Yoshiki Sawa; Mitsuru Akashi

doi:10.1002/adhm.201500065

Dynamic nano-interfaces enable harvesting of functional 3D-engineered tissues

Adv Healthc Mater. 2015 Jun 3;4(8):1164-8. doi: 10.1002/adhm.201500065. Epub 2015 Feb 27.

Authors

Akihiro Nishiguchi¹, Michiya Matsusaki¹, Shigeru Miyagawa², Yoshiki Sawa², Mitsuru Akashi¹

Affiliations

¹ Department of Applied Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamada-oka, Suita, Osaka, 565-0871, Japan.
² Department of Surgery, Division of Cardiovascular Surgery, Graduate School of Medicine, Osaka University, 2-2 Yamada-oka, Suita, Osaka, 565-0087, Japan.

PMID: 25728509
DOI: 10.1002/adhm.201500065

Abstract

Functional 3D-engineered tissues are successfully harvested from a substrate using stimuli-responsive hydrogel films with dynamic nano-interface. The dynamic wettability control at the interfaces allows cellular detachment, leading to tissue harvesting without serious damage and remaining polymers. This method can be applied to various types of organs and used for tissue transplantation in regenerative medicine.

Keywords: cell adhesion; dynamic interfaces; gels; thin films; tissue engineering.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Adhesion
Cell Survival / drug effects
Heart Diseases / therapy
Human Umbilical Vein Endothelial Cells
Humans
Hydrogel, Polyethylene Glycol Dimethacrylate / chemistry
Induced Pluripotent Stem Cells / cytology
Induced Pluripotent Stem Cells / metabolism
Microscopy, Confocal
Nanoparticles / chemistry*
Polyglutamic Acid / chemistry
Polymers / chemistry
Regenerative Medicine / methods
Tissue Engineering / methods*

Substances

Polymers
Polyglutamic Acid
Hydrogel, Polyethylene Glycol Dimethacrylate