The distribution of neurons exhibiting somatostatin (SRIF)-, neuropeptide Y (NPY)-, beta-endorphin- and neurotensin (NT)-like immunoreactivity within the infundibular nucleus (NI) of the sheep, and the extent of coexistence of the above peptides within individual neurons of the NI were investigated with immunocytochemical techniques. Our results show that the above neurochemical types of neurons exhibit specific and largely non-overlapping patterns of distribution within the NI of the sheep. Furthermore, the coexistence of these peptides within neurons of the NI is very limited, as from all possible permutations checked, only SRIF and NPY were found together in a small number of cells.