Non-derivatized glycan analysis by reverse phase liquid chromatography and ion mobility-mass spectrometry

Nichole M Lareau; Jody C May; John A McLean

doi:10.1039/c5an00152h

Non-derivatized glycan analysis by reverse phase liquid chromatography and ion mobility-mass spectrometry

Analyst. 2015 May 21;140(10):3335-8. doi: 10.1039/c5an00152h. Epub 2015 Mar 4.

Authors

Nichole M Lareau¹, Jody C May, John A McLean

Affiliation

¹ Department of Chemistry, Center for Innovative Technology, Institute of Chemical Biology, and Institute for Integrative Biosystems Research and Education, Vanderbilt University, 7330 Stevenson Center, Nashville, TN 37235, USA. john.a.mclean@vanderbilt.edu.

Abstract

A simple method for the analysis of non-derivatized glycans using a reverse phase column on a liquid chromatography-ion mobility-mass spectrometry (LC-IM-MS) instrument. The methodology supports both glycomic and proteomic work flows without the necessity of switching columns.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Chromatography, Reverse-Phase / methods*
Glycomics
Mass Spectrometry / methods*
Polysaccharides / analysis*
Polysaccharides / chemistry
Proteomics

Substances

Polysaccharides

Abstract

Publication types

MeSH terms

Substances

Grants and funding