NDM-1-producing Enterobacteriaceae in a teaching hospital in Shanghai, China: IncX3-type plasmids may contribute to the dissemination of blaNDM-1

Hongping Qu; Xiaoli Wang; Yuxing Ni; Jialin Liu; Ruoming Tan; Jie Huang; Lei Li; Jingyong Sun

doi:10.1016/j.ijid.2015.02.020

NDM-1-producing Enterobacteriaceae in a teaching hospital in Shanghai, China: IncX3-type plasmids may contribute to the dissemination of blaNDM-1

Int J Infect Dis. 2015 May:34:8-13. doi: 10.1016/j.ijid.2015.02.020. Epub 2015 Mar 2.

Authors

Hongping Qu¹, Xiaoli Wang¹, Yuxing Ni², Jialin Liu³, Ruoming Tan¹, Jie Huang¹, Lei Li⁴, Jingyong Sun⁵

Affiliations

¹ Department of Critical Care Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, No.197 Ruijin ER Road, Shanghai 200025, China.
² Department of Clinical Microbiology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, No.197 Ruijin ER Road, Shanghai 200025, China.
³ Department of Pulmonary Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, No.197 Ruijin ER Road, Shanghai 200025, China.
⁴ Department of Critical Care Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, No.197 Ruijin ER Road, Shanghai 200025, China. Electronic address: Lileiys1023@yeah.net.
⁵ Department of Clinical Microbiology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, No.197 Ruijin ER Road, Shanghai 200025, China. Electronic address: 13671578899@126.com.

PMID: 25743762
DOI: 10.1016/j.ijid.2015.02.020

Abstract

Objectives: To provide the epidemiological dissemination and the genetic characteristics of bla(NDM-1) in a teaching hospital in Shanghai, China.

Methods: Here, the carbapenemase genes of 114 CRE isolates were evaluated by polymerase chain reaction (PCR). Clonal relatedness was assessed by pulsed-field gel electrophoresis (PFGE). Conjugation experiments and Southern blot hybridization were performed to determine the transferability of plasmids. Then plasmids were completely sequenced by the shotgun method.

Results: Two Klebsiella pneumoniae strains (RJA1227 and RJF866) and one Raoultella planticola strain (RJA274) were identified as NDM-1 positive. The two K. pneumoniae isolates belonged to ST11 and exhibited highly similar PFGE patterns. Shotgun sequencing showed that plasmid pRJF866 (ca. 110 kb) contained genes associated with the IncFII-FIB group and was highly similar to plasmid pKOX_NDM1. RJA274 (ca. 50 kb) harbored bla(NDM-1) on an IncX3 plasmid, which was nearly identical to plasmid pNDM-HN380 except that part of the ISAba125 element is missing.

Conclusion: This is the first report of NDM-1-positive Enterobacteriaceae from Shanghai, China. IncX3 plasmids, reported in various species in the United Arab Emirates and China, may contribute to the dissemination of bla(NDM-1.). More attention should be devoted to monitoring the dissemination of the NDM-1 gene due to its potential horizontal transfer via mobile genetic elements.

Keywords: Carbapenem-resistant Enterobacteriaceae; Dissemination; NDM-1; Plasmid.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Proteins / genetics*
China / epidemiology
Conjugation, Genetic
Cross Infection / epidemiology*
Cross Infection / microbiology
Electrophoresis, Gel, Pulsed-Field
Enterobacteriaceae / genetics*
Enterobacteriaceae / isolation & purification
Enterobacteriaceae Infections / epidemiology*
Gene Order
Hospitals, Teaching
Humans
Klebsiella Infections / epidemiology*
Klebsiella Infections / microbiology
Klebsiella pneumoniae / genetics*
Klebsiella pneumoniae / isolation & purification
Male
Plasmids / genetics
Plasmids / isolation & purification
United Arab Emirates
Young Adult
beta-Lactamases / genetics*

Substances

Bacterial Proteins
beta-Lactamases
beta-lactamase NDM-1
carbapenemase