Application of continuous-flow liquid chromatography/fast-atom bombardment mass spectrometry to the analysis of diagnostic acylcarnitines in human urine

D L Norwood; N Kodo; D S Millington

doi:10.1002/rcm.1290021205

Application of continuous-flow liquid chromatography/fast-atom bombardment mass spectrometry to the analysis of diagnostic acylcarnitines in human urine

Rapid Commun Mass Spectrom. 1988 Dec;2(12):269-72. doi: 10.1002/rcm.1290021205.

Authors

D L Norwood¹, N Kodo, D S Millington

Affiliation

¹ Duke University Medical Center, Department of Pediatrics, Durham, North Carolina 27710.

PMID: 2577839
DOI: 10.1002/rcm.1290021205

Abstract

Acylcarnitine profiles of diagnostic value were generated from the equivalent of 0.1 microL of raw urine using the continuous-flow liquid chromatography/fast-atom bombardment mass spectrometry (LC/FAB-MS) interface for sample introduction. Further analysis was accomplished by gradient LC/MS using a commercially available packed fused-silica microbore column.

MeSH terms

Carnitine / analogs & derivatives*
Carnitine / urine
Chromatography, Liquid / methods
Fatty Acid Desaturases / deficiency
Hemiterpenes
Humans
Pentanoic Acids / blood
Propionates / blood
Spectrometry, Mass, Fast Atom Bombardment / methods

Substances

Hemiterpenes
Pentanoic Acids
Propionates
isovaleric acid
Fatty Acid Desaturases
propionic acid
Carnitine