Differential expression of miRNAs and their relation to active tuberculosis

Zhihong Xu; Aiping Zhou; Jinjing Ni; Qiufen Zhang; Ying Wang; Jie Lu; Wenjuan Wu; Petros C Karakousis; Shuihua Lu; Yufeng Yao

doi:10.1016/j.tube.2015.02.043

Differential expression of miRNAs and their relation to active tuberculosis

Tuberculosis (Edinb). 2015 Jul;95(4):395-403. doi: 10.1016/j.tube.2015.02.043. Epub 2015 Mar 6.

Authors

Zhihong Xu¹, Aiping Zhou², Jinjing Ni¹, Qiufen Zhang¹, Ying Wang³, Jie Lu⁴, Wenjuan Wu⁵, Petros C Karakousis⁶, Shuihua Lu⁷, Yufeng Yao⁸

Affiliations

¹ Laboratory of Bacterial Pathogenesis, Department of Microbiology and Immunology, Institutes of Medical Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.
² Laboratory of Bacterial Pathogenesis, Department of Microbiology and Immunology, Institutes of Medical Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China; Department of Laboratory Medicine, East Hospital Affiliated to Tongji University, Shanghai 200120, China.
³ Shanghai Institute of Immunology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.
⁴ Shanghai Ruijin Hospital, Shanghai 200025, China.
⁵ Department of Laboratory Medicine, East Hospital Affiliated to Tongji University, Shanghai 200120, China.
⁶ Center for Tuberculosis Research, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.
⁷ Shanghai Public Health Clinical Center, Shanghai 201508, China. Electronic address: tubercle@shaphc.org.
⁸ Laboratory of Bacterial Pathogenesis, Department of Microbiology and Immunology, Institutes of Medical Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China; Department of Laboratory Medicine, East Hospital Affiliated to Tongji University, Shanghai 200120, China. Electronic address: yfyao@sjtu.edu.cn.

PMID: 25936536
DOI: 10.1016/j.tube.2015.02.043

Abstract

The aim of this work was to screen miRNA signatures dysregulated in tuberculosis to improve our understanding of the biological role of miRNAs involved in the disease. Datasets deposited in publically available databases from microarray studies on infectious diseases and malignancies were retrieved, screened, and subjected to further analysis. Effect sizes were combined using the inverse-variance model and between-study heterogeneity was evaluated by the random effects model. 35 miRNAs were differentially expressed (12 up-regulated, 23 down-regulated; p < 0.05) by combining 15 datasets of tuberculosis and other infectious diseases. 15 miRNAs were found to be significantly differentially regulated (7 up-regulated, 8 down-regulated; p < 0.05) by combining 53 datasets of tuberculosis and malignancies. Most of the miRNA signatures identified in this study were found to be involved in immune responses and metabolism. Expression of these miRNA signatures in serum samples from TB subjects (n = 11) as well as healthy controls (n = 10) was examined by TaqMan miRNA array. Taken together, the results revealed differential expression of miRNAs in TB, but available datasets are limited and these miRNA signatures should be validated in future studies.

Keywords: Microarray; Tuberculosis; microRNA.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Adolescent
Adult
Case-Control Studies
Computational Biology
Databases, Genetic
Female
Gene Expression Profiling* / methods
Genetic Markers
Humans
Male
MicroRNAs / blood
MicroRNAs / genetics*
Middle Aged
Oligonucleotide Array Sequence Analysis
Tuberculosis / blood
Tuberculosis / diagnosis
Tuberculosis / genetics*
Tuberculosis / microbiology
Young Adult

Substances

Genetic Markers
MicroRNAs

Abstract

Publication types

MeSH terms

Substances

Grants and funding