Complete sequence of the coding region of human elongation factor 2 (EF-2) by enzymatic amplification of cDNA from human ovarian granulosa cells

Biol Chem Hoppe Seyler. 1989 Oct;370(10):1071-5. doi: 10.1515/bchm3.1989.370.2.1071.

Abstract

The use of two primers allowed the specific enzymatic amplification of elongation factor 2 starting with total double-stranded cDNA from human ovarian granulosa cells. The amplified DNA fragment with a length of 1765 bp was restricted and sequenced by the shot gun approach. From the sequences obtained from the amplified fragment and the cDNA insert of pHGR81 [Rapp et al. (1988) Biol. Chem. Hoppe-Seyler 369, 247-250] respectively, the DNA sequence containing the complete coding as well as the 3'-untranslated region was assembled.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Cricetinae
  • DNA / analysis*
  • Female
  • Granulosa Cells / analysis*
  • Humans
  • Molecular Sequence Data
  • Nucleic Acid Amplification Techniques*
  • Peptide Elongation Factor 2
  • Peptide Elongation Factors / genetics*
  • Rats
  • Sequence Homology, Nucleic Acid

Substances

  • Peptide Elongation Factor 2
  • Peptide Elongation Factors
  • DNA