Lysosome targeting fluorescence probe for imaging intracellular thiols

Dnyaneshwar Kand; Tanmoy Saha; Mayurika Lahiri; Pinaki Talukdar

doi:10.1039/c5ob00889a

Lysosome targeting fluorescence probe for imaging intracellular thiols

Org Biomol Chem. 2015 Aug 14;13(30):8163-8. doi: 10.1039/c5ob00889a.

Authors

Dnyaneshwar Kand¹, Tanmoy Saha, Mayurika Lahiri, Pinaki Talukdar

Affiliation

¹ Department of Chemistry, Indian Institute of Science Education and Research Pune, Pune 411008, India. ptalukdar@iiserpune.ac.in.

PMID: 26140677
DOI: 10.1039/c5ob00889a

Abstract

A BODIPY-based fluorescence turn-on probe, exhibiting high selectivity and sensitivity towards intracellular thiols with excellent lysosomal localization is reported. The probe displayed fast response towards biothiols in aqueous solution. Localization of the probe in lysosome was demonstrated by intracellular colocalization studies with the aid of LysoSensor Green.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromatography, High Pressure Liquid
Fluorescence
Fluorescent Dyes / chemical synthesis
Fluorescent Dyes / chemistry
Fluorescent Dyes / metabolism*
HeLa Cells
Humans
Imaging, Three-Dimensional*
Intracellular Space / chemistry*
Kinetics
Lysosomes / metabolism*
Sulfhydryl Compounds / metabolism*

Substances

Fluorescent Dyes
Sulfhydryl Compounds