Gene Transcriptional and Metabolic Profile Changes in Mimetic Aging Mice Induced by D-Galactose

Yue-Yue Zhou; Xiong-Fei Ji; Jian-Ping Fu; Xiao-Juan Zhu; Rong-Hua Li; Chang-Kao Mu; Chun-Lin Wang; Wei-Wei Song

doi:10.1371/journal.pone.0132088

Gene Transcriptional and Metabolic Profile Changes in Mimetic Aging Mice Induced by D-Galactose

PLoS One. 2015 Jul 15;10(7):e0132088. doi: 10.1371/journal.pone.0132088. eCollection 2015.

Authors

Yue-Yue Zhou¹, Xiong-Fei Ji¹, Jian-Ping Fu¹, Xiao-Juan Zhu¹, Rong-Hua Li¹, Chang-Kao Mu¹, Chun-Lin Wang¹, Wei-Wei Song¹

Affiliation

¹ Key Laboratory of the Ministry of Education for Applied Marine Biotechnology, Ningbo University, Ningbo, China; Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture, Ningbo University, Ningbo, China.

Abstract

D-galactose injection has been shown to induce many changes in mice that represent accelerated aging. This mouse model has been widely used for pharmacological studies of anti-aging agents. The underlying mechanism of D-galactose induced aging remains unclear, however, it appears to relate to glucose and 1ipid metabolic disorders. Currently, there has yet to be a study that focuses on investigating gene expression changes in D-galactose aging mice. In this study, integrated analysis of gas chromatography/mass spectrometry-based metabonomics and gene expression profiles was used to investigate the changes in transcriptional and metabolic profiles in mimetic aging mice injected with D-galactose. Our findings demonstrated that 48 mRNAs were differentially expressed between control and D-galactose mice, and 51 potential biomarkers were identified at the metabolic level. The effects of D-galactose on aging could be attributed to glucose and 1ipid metabolic disorders, oxidative damage, accumulation of advanced glycation end products (AGEs), reduction in abnormal substance elimination, cell apoptosis, and insulin resistance.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aging / genetics*
Animals
Biomarkers / metabolism
Galactose / pharmacology*
Gas Chromatography-Mass Spectrometry
Liver / drug effects
Liver / metabolism
Metabolome / drug effects*
Metabolomics*
Mice, Inbred ICR
Multivariate Analysis
Oligonucleotide Array Sequence Analysis
RNA, Messenger / genetics
RNA, Messenger / metabolism
Real-Time Polymerase Chain Reaction
Reproducibility of Results
Transcription, Genetic / drug effects*

Substances

Biomarkers
RNA, Messenger
Galactose

Grants and funding

Funding provided by 41176124, National Natural Science Foundation of China to WWS, 41206114, National Natural Science Foundation of China to WWS, LQ12C19002, Zhejiang Province Science and Technology Hall to WWS, ZX2013000403, Zhejiang Province Science and Technology Hall to WWS, Y201224605, Zhejiang Province Department of Education to WWS, XKL11D2102, Ningbo University to WWS, XYL12005, Ningbo University to WWS, and K. C. Wong Magana Fund, Ningbo University to WWS. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.