ATP4 and ciliation in the neuroectoderm and endoderm of Xenopus embryos and tadpoles

Peter Walentek; Cathrin Hagenlocher; Tina Beyer; Christina Müller; Kerstin Feistel; Axel Schweickert; Richard M Harland; Martin Blum

doi:10.1016/j.dib.2015.04.003

ATP4 and ciliation in the neuroectoderm and endoderm of Xenopus embryos and tadpoles

Data Brief. 2015 Apr 20:4:22-31. doi: 10.1016/j.dib.2015.04.003. eCollection 2015 Sep.

Authors

Peter Walentek¹, Cathrin Hagenlocher², Tina Beyer², Christina Müller², Kerstin Feistel², Axel Schweickert², Richard M Harland³, Martin Blum²

Affiliations

¹ Institute of Zoology, University of Hohenheim, Garbenstrasse 30, 70593 Stuttgart, Germany ; Department of Molecular and Cell Biology, Center for Integrative Genomics, University of California at Berkeley, Berkeley, California 94720, USA.
² Institute of Zoology, University of Hohenheim, Garbenstrasse 30, 70593 Stuttgart, Germany.
³ Department of Molecular and Cell Biology, Center for Integrative Genomics, University of California at Berkeley, Berkeley, California 94720, USA.

Abstract

During gastrulation and neurulation, foxj1 expression requires ATP4a-dependent Wnt/β-catenin signaling for ciliation of the gastrocoel roof plate (Walentek et al. Cell Rep. 1 (2012) 516-527.) and the mucociliary epidermis (Walentek et al. Dev. Biol. (2015)) of Xenopus laevis embryos. These data suggested that ATP4a and Wnt/β-catenin signaling regulate foxj1 throughout Xenopus development. Here we analyzed whether foxj1 expression was also ATP4a-dependent in other ciliated tissues of the developing Xenopus embryo and tadpole. We found that in the floor plate of the neural tube ATP4a-dependent canonical Wnt signaling was required for foxj1 expression, downstream of or in parallel to Hedgehog signaling. In the developing tadpole brain, ATP4-function was a prerequisite for the establishment of cerebrospinal fluid flow. Furthermore, we describe foxj1 expression and the presence of multiciliated cells in the developing tadpole gastrointestinal tract. Our work argues for a general requirement of ATP4-dependent Wnt/β-catenin signaling for foxj1 expression and motile ciliogenesis throughout Xenopus development.

Keywords: ATP4a; Cilia; Gastric H+/K+ATPase; Wnt signaling; Xenopus.

Grants and funding

R01 GM042341/GM/NIGMS NIH HHS/United States