CRISPR EATING on a Low Budget

Hatice S Kaya-Okur; Andrew S Belmont

doi:10.1016/j.devcel.2015.07.013

CRISPR EATING on a Low Budget

Dev Cell. 2015 Aug 10;34(3):253-4. doi: 10.1016/j.devcel.2015.07.013.

Authors

Hatice S Kaya-Okur¹, Andrew S Belmont²

Affiliations

¹ Department of Cell and Developmental Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
² Department of Cell and Developmental Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA. Electronic address: asbel@illinois.edu.

PMID: 26267392
DOI: 10.1016/j.devcel.2015.07.013

Abstract

In this issue of Developmental Cell, Lane et al. (2015) describe a simplified, inexpensive technique named "CRISPR EATING" for generating complex guide RNA libraries suitable for CRISPR/Cas9-based applications ranging from genome visualization and manipulation to genetic screens.

Publication types

Comment

MeSH terms

Animals
Bacterial Proteins*
Clustered Regularly Interspaced Short Palindromic Repeats / genetics*
Endonucleases*
Gene Library*
RNA / genetics*

Substances

Bacterial Proteins
RNA
Endonucleases

Grants and funding

R01 GM058460/GM/NIGMS NIH HHS/United States