Abstract
MicroRNAs exert their functions by mainly regulating coding genes or long non-coding RNA expression. In the present study, we reported that hsa-miR-1 was down-regulated in breast cancer tissues. Restoration of miR-1 in breast cancer cells inhibited proliferation, motility and increased apoptosis in vitro. MiR-1 functioned as a tumor suppressor by targeting K-RAS and MALAT1. In addition, the effects of up-regulation of miR-1 were similar to that of silencing K-RAS and MALAT1 in breast cancer cells. In vivo study indicated that restoration of miR-1 inhibited tumor growth and metastasis. Patients with low miR-1 expression had poorer overall survival time than those with high miR-1 expression. Our findings emphasized the potential role of miR-1 as tumor suppressive miRNA in breast cancer.
Keywords:
K-RAS; MALAT1; miR-1.
Copyright © 2015 Elsevier B.V. All rights reserved.
MeSH terms
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3' Untranslated Regions
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Adult
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Aged
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Animals
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Apoptosis / genetics
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Base Sequence
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Binding Sites
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Biomarkers, Tumor
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Breast Neoplasms / genetics*
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Breast Neoplasms / metabolism
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Breast Neoplasms / pathology
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Cell Line, Tumor
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Cell Movement / genetics
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Cell Proliferation / genetics
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Cell Transformation, Neoplastic / genetics*
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Disease Models, Animal
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Down-Regulation
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Ectopic Gene Expression
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Female
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Gene Expression Profiling
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Gene Expression Regulation, Neoplastic*
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Humans
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MicroRNAs / chemistry
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MicroRNAs / genetics*
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Middle Aged
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Neoplasm Metastasis
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Neoplasm Staging
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Proto-Oncogene Proteins p21(ras) / chemistry
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Proto-Oncogene Proteins p21(ras) / genetics*
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RNA Interference*
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RNA, Long Noncoding / chemistry
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RNA, Long Noncoding / genetics*
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RNA, Messenger / chemistry
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RNA, Messenger / genetics
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Tumor Burden / genetics
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Xenograft Model Antitumor Assays
Substances
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3' Untranslated Regions
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Biomarkers, Tumor
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KRAS protein, human
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MALAT1 long non-coding RNA, human
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MIRN1 microRNA, human
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MicroRNAs
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RNA, Long Noncoding
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RNA, Messenger
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Proto-Oncogene Proteins p21(ras)