Visualizing Cytoophidia Expression in Drosophila Follicle Cells via Immunohistochemistry

Methods Mol Biol. 2015:1328:179-89. doi: 10.1007/978-1-4939-2851-4_13.

Abstract

We describe a user-friendly immunohistochemical approach for the detection of protein localization in Drosophila ovaries, here focusing on CTP synthase. This approach mainly uses fluorescently labeled antibodies to detect single, double, or multiple antigens. We provide a step-by-step protocol with detailed notes and tips, a simplified method that can also be adapted to detect protein localization beyond Drosophila ovaries.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies / chemistry
  • Carbon-Nitrogen Ligases / biosynthesis*
  • Carbon-Nitrogen Ligases / isolation & purification
  • Drosophila melanogaster
  • Female
  • Immunohistochemistry / methods*
  • Ovarian Follicle / metabolism
  • Ovarian Follicle / ultrastructure*

Substances

  • Antibodies
  • Carbon-Nitrogen Ligases
  • CTP synthetase