Characteristics of Human Turbinate-Derived Mesenchymal Stem Cells Are Not Affected by Allergic Condition of Donor

Se Hwan Hwang; Hye Kyung Cho; Sang Hi Park; WeonSun Lee; Hee Jin Lee; Dong Chang Lee; Sun Hwa Park; Mi Hyun Lim; Sang A Back; Byeong Gon Yun; Dong Il Sun; Jun Myung Kang; Sung Won Kim

doi:10.1371/journal.pone.0138041

Characteristics of Human Turbinate-Derived Mesenchymal Stem Cells Are Not Affected by Allergic Condition of Donor

PLoS One. 2015 Sep 16;10(9):e0138041. doi: 10.1371/journal.pone.0138041. eCollection 2015.

Authors

Affiliations

¹ Department of Otolaryngology-Head and Neck Surgery, The Catholic University of Korea, College of Medicine, Seoul, Korea.
² Department of Pediatrics, Graduate School of Medicine, Gachon University, Incheon, Korea.
³ Institute of Clinical Medicine Research, College of Medicine, Catholic University of Korea, Seoul, Korea.
⁴ Department of Otolaryngology-Head and Neck Surgery, The Catholic University of Korea, College of Medicine, Seoul, Korea; Department of biomedical science, The Catholic University of Korea, College of Medicine, Seoul, Korea.

Abstract

The characteristics of mesenchymal stem cells (MSCs) derived from human turbinates (hTMSCs) have not been investigated in allergic rhinitis. We evaluated the influence of allergic state of the donor on the characteristics, proliferation, and differentiation potential of hTMSCs, compared with hTMSCs derived from non-allergic patients. hTMSCs were isolated from five non-allergic and five allergic patients. The expression of toll-like receptors (TLRs) in hTMSCs was measured by FACS, and cell proliferation was measured using a cell counting kit. Cytokine secretion was analyzed using multiplex immunoassays. The osteogenic, chondrogenic, and adipogenic differentiation potentials of hTMSCs were evaluated by histology and gene expression analysis. In allergic patients, FACS analysis showed that TLR3 and TLR4 were more highly expressed on the surface of hTMSCs than TLR2 and TLR5. The proliferation of hTMSCs was not influenced by the presence of TLR priming. The expression of IL-6, IL-8, IL-12, IP-10, and RANTES was upregulated after the TLR4 priming. The differentiation potential of hTMSCs was not influenced by TLR priming. These characteristics of hTMSCs were similar to those of hTMSCs from non-allergic patients. We conclude that the allergic condition of the donor does not influence TLR expression, proliferation, or immunomodulatory potential of hTMSCs.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Allergens / immunology*
Antigens, Surface / immunology*
Antigens, Surface / metabolism
Blotting, Western
Cell Differentiation
Cell Proliferation
Cells, Cultured
Cytokines / genetics
Cytokines / metabolism
Flow Cytometry
Humans
Mesenchymal Stem Cells / immunology
Mesenchymal Stem Cells / metabolism
Mesenchymal Stem Cells / pathology*
RNA, Messenger / genetics
Real-Time Polymerase Chain Reaction
Reverse Transcriptase Polymerase Chain Reaction
Rhinitis, Allergic / immunology
Rhinitis, Allergic / metabolism
Rhinitis, Allergic / pathology*
Rhinitis, Atrophic / immunology
Rhinitis, Atrophic / metabolism
Rhinitis, Atrophic / pathology*
Toll-Like Receptors / genetics
Toll-Like Receptors / metabolism
Turbinates / immunology
Turbinates / metabolism
Turbinates / pathology*

Substances

Allergens
Antigens, Surface
Cytokines
RNA, Messenger
Toll-Like Receptors

Grants and funding

The authors wish to acknowledge the financial support of the Catholic Medical Center Research Foundation made in the program year of 2014, the Institute of Clinical Medicine Research of Bucheon St. Mary's Hospital, Research Fund, 2015 and the National Research Foundation of Korea (NRF), funded by the Ministry of Science, ICT, and Future Planning (2014R1A2A2A01004325), and the Korea Health Industry Development Institute, funded by the Ministry of Health and Welfare (HI14C3228). However, the funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.