First report of bacterial community from a Bat Guano using Illumina next-generation sequencing

Surajit De Mandal; Zothansanga; Amritha Kumari Panda; Satpal Singh Bisht; Nachimuthu Senthil Kumar

doi:10.1016/j.gdata.2015.04.001

First report of bacterial community from a Bat Guano using Illumina next-generation sequencing

Genom Data. 2015 Apr 9:4:99-101. doi: 10.1016/j.gdata.2015.04.001. eCollection 2015 Jun.

Authors

Surajit De Mandal¹, Zothansanga¹, Amritha Kumari Panda¹, Satpal Singh Bisht¹, Nachimuthu Senthil Kumar¹

Affiliation

¹ Department of Biotechnology, Mizoram University, Aizawl 796004, Mizoram, India.

Abstract

V4 hypervariable region of 16S rDNA was analyzed for identifying the bacterial communities present in Bat Guano from the unexplored cave - Pnahkyndeng, Meghalaya, Northeast India. Metagenome comprised of 585,434 raw Illumina sequences with a 59.59% G+C content. A total of 416,490 preprocessed reads were clustered into 1282 OTUs (operational taxonomical units) comprising of 18 bacterial phyla. The taxonomic profile showed that the guano bacterial community is dominated by Chloroflexi, Actinobacteria and Crenarchaeota which account for 70.73% of all sequence reads and 43.83% of all OTUs. Metagenome sequence data are available at NCBI under the accession no. SRP051094. This study is the first to characterize Bat Guano bacterial community using next-generation sequencing approach.

Keywords: Bat Guano; Illumina; Metagenome.