Identification and Expression Analysis of a Novel HbCIPK2-Interacting Ferredoxin from Halophyte H. brevisubulatum

PLoS One. 2015 Dec 4;10(12):e0144132. doi: 10.1371/journal.pone.0144132. eCollection 2015.

Abstract

Ferredoxin is a small iron-sulfer protein involved in various one-eletron transfer pathways. Little is known about how ferredoxin is regulated to distribute electron under abiotic stress. Our previous study has showed that HbCIPK2 conferred salinity and drought tolerance. Thus, we hypothesized that HbCIPK2 could mediate the activities of interacting partners as a signal transducer. In this report, we identified a novel HbCIPK2-interacting ferredoxin (HbFd1) from halophyte Hordeum brevisubulatum by yeast two-hybrid screens, confirmed this interaction by BiFC in vivo and CoIP in vitro, and presented the expression pattern of HbFd1. HbFd1 was down-regulated under salinity and cold stress but up-regulated under PEG stress, its expression showed tissue-specific, mainly in shoot chloroplast, belonging to leaf-type subgroup. Moreover, HbCIPK2 could recruit HbFd1 to the nucleus for their interaction. The C-terminal segment in HbFd1 protein was involved in the interaction with HbCIPK2. These results provided insight into the connection between CBL-CIPK signaling network and Fd-dependent metabolic pathways.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Nucleus / metabolism
  • Evolution, Molecular
  • Ferredoxins / genetics*
  • Ferredoxins / metabolism*
  • Gene Expression Regulation, Plant
  • HEK293 Cells
  • Hordeum / genetics
  • Hordeum / growth & development*
  • Hordeum / metabolism*
  • Humans
  • Metabolic Networks and Pathways
  • Organ Specificity
  • Plant Proteins / genetics
  • Plant Proteins / metabolism
  • Protein Serine-Threonine Kinases / metabolism*
  • Salt-Tolerant Plants / genetics
  • Salt-Tolerant Plants / metabolism
  • Two-Hybrid System Techniques

Substances

  • Ferredoxins
  • Plant Proteins
  • Protein Serine-Threonine Kinases

Grants and funding

This work was supported by National Natural Science Foundation of China to RL (31271785), http://www.nsfc.gov.cn/, National Transgenic Major Program of China to JW (2014ZX0800917B), http://www.nmp.gov.cn/, Beijing Natural Science Foundation to RL (5132010), http://www.bjnsf.org/, Special Grant for Innovative Ability Improvement from Beijing Academy of Agriculture and Forestry Sciences to JW and RL (KJCX20140202), http://www.baafs.net.cn/ and Grant for Institute Innovation Team from Beijing Academy of Agriculture and Forestry Sciences to RL, http://www.baafs.net.cn/. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.