Angiogenic Type I Collagen Extracellular Matrix Integrated with Recombinant Bacteriophages Displaying Vascular Endothelial Growth Factors

Adv Healthc Mater. 2016 Jan 21;5(2):205-12. doi: 10.1002/adhm.201500534. Epub 2015 Dec 7.

Abstract

Here, a growth-factor-integrated natural extracellular matrix of type I collagen is presented that induces angiogenesis. The developed matrix adapts type I collagen nanofibers integrated with synthetic colloidal particles of recombinant bacteriophages that display vascular endothelial growth factor (VEGF). The integration is achieved during or after gelation of the type I collagen and the matrix enables spatial delivery of VEGF into a desired region. Endothelial cells that contact the VEGF are found to invade into the matrix to form tube-like structures both in vitro and in vivo, proving the angiogenic potential of the matrix.

Keywords: angiogenesis; bacteriophages; collagen; growth factors, VEGF.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacteriophages / metabolism*
  • Cell Surface Display Techniques
  • Collagen Type I / pharmacology*
  • Enzyme-Linked Immunosorbent Assay
  • Extracellular Matrix / drug effects
  • Extracellular Matrix / metabolism*
  • Fluorescent Antibody Technique
  • Humans
  • Mice, Inbred BALB C
  • Microscopy, Fluorescence
  • Nanofibers / chemistry
  • Neovascularization, Physiologic / drug effects*
  • Protein Engineering
  • Rats
  • Recombination, Genetic / genetics*
  • Vascular Endothelial Growth Factors / metabolism*

Substances

  • Collagen Type I
  • Vascular Endothelial Growth Factors