Complete Sequences of Multidrug Resistance Plasmids Bearing rmtD1 and rmtD2 16S rRNA Methyltransferase Genes

Antimicrob Agents Chemother. 2016 Jan 4;60(3):1928-31. doi: 10.1128/AAC.02562-15.

Abstract

Complete nucleotide sequences were determined for two plasmids bearing rmtD group 16S rRNA methyltransferase genes. pKp64/11 was 78 kb in size, belonged to the IncL/M group, and harbored blaTEM-1b, sul1, qacEΔ1, dfrA22, and rmtD1 across two multidrug resistance regions (MRRs). pKp368/10 was 170 kb in size, belonged to the IncA/C group, and harbored acrB, sul1, qacEΔ1, ant(3″)-Ia, aac(6')-Ib, cat, rmtD2, and blaCTX-M-8 across three MRRs. The rmtD-containing regions shared a conserved motif, suggesting a common origin for the two rmtD alleles.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / pharmacology
  • Bacterial Proteins / genetics
  • Base Sequence
  • Drug Resistance, Multiple, Bacterial / genetics*
  • Escherichia coli / drug effects
  • Escherichia coli / genetics
  • Klebsiella pneumoniae / drug effects
  • Klebsiella pneumoniae / genetics
  • Membrane Transport Proteins / genetics*
  • Methyltransferases / genetics*
  • Microbial Sensitivity Tests
  • Plasmids / genetics*
  • RNA, Ribosomal, 16S / genetics*
  • Sequence Analysis, RNA
  • beta-Lactamases / genetics*

Substances

  • Anti-Bacterial Agents
  • Bacterial Proteins
  • Membrane Transport Proteins
  • RNA, Ribosomal, 16S
  • Methyltransferases
  • beta-Lactamases
  • beta-lactamase TEM-1