[Expression pattern of membrane type-1 matrix metalloproteinase in primary breast carcinomas]

Nan Fang Yi Ke Da Xue Xue Bao. 2016 Jan;36(1):94-7.
[Article in Chinese]

Abstract

Objective: To examine the expression pattern of membrane type-1 matrix metalloproteinase (MT1-MMP) in breast carcinomas.

Methods: Forty-three breast cancer tissues were collected and examined for MT1-MMP protein and mRNA expressions using immunohistochemistry, semi-quantitative RT-PCR and in situ hybridization.

Results: Immunohistochemistry of the breast cancer specimens showed MT1-MMP immunoreactivity on the cancer cell membrane. MT1-MMP mRNA was located in the stromal cells surrounding the breast cancer nest as shown by in situ hybridization. MT1-MMP mRNA expression was detected in all of the carcinomas, but its level was significantly lower in immunohistochemically negative specimens than in positive ones (0.547=0.0886 vs 0.759=0.0802, Plt;0.01).

Conclusion: MT1-MMP is very likely produced by stromal cells surrounding the breast cancer nest and anchored on the cell membrane after activation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Breast Neoplasms / genetics
  • Breast Neoplasms / metabolism*
  • Female
  • Humans
  • Immunohistochemistry
  • In Situ Hybridization
  • Matrix Metalloproteinase 14 / genetics
  • Matrix Metalloproteinase 14 / metabolism*
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Tumor Cells, Cultured

Substances

  • RNA, Messenger
  • MMP14 protein, human
  • Matrix Metalloproteinase 14