Reactive Oxygen Species Imaging in a Mouse Model of Inflammatory Bowel Disease

Laura Bronsart; Linh Nguyen; Aida Habtezion; Christopher Contag

doi:10.1007/s11307-016-0934-0

Reactive Oxygen Species Imaging in a Mouse Model of Inflammatory Bowel Disease

Mol Imaging Biol. 2016 Aug;18(4):473-8. doi: 10.1007/s11307-016-0934-0.

Authors

Laura Bronsart^{1

2}, Linh Nguyen³, Aida Habtezion³, Christopher Contag^{4

5}

Affiliations

¹ Department of Biology, Stanford University, 318 Campus Drive, Stanford, CA, 94305, USA.
² Department of Pediatrics, Stanford University, 318 Campus Drive, Stanford, CA, 94305, USA.
³ Division of Gastroenterology and Hepatology, Department of Medicine, Stanford University, 318 Campus Drive, Stanford, CA, 94305, USA.
⁴ Department of Pediatrics, Stanford University, 318 Campus Drive, Stanford, CA, 94305, USA. ccontag@stanford.edu.
⁵ Departments of Radiology, Microbiology & Immunology, Stanford University, 318 Campus Drive, Stanford, CA, 94305, USA. ccontag@stanford.edu.

Abstract

Purpose: Reactive oxygen species (ROS) are important contributors to inflammatory bowel disease (IBD); however, there are insufficient tools for their in vivo evaluation.

Procedures: To determine if a chemiluminescent ROS reporter, coelenterazine, would be a useful tool for the detection of immune cell activation, the macrophage cell line (RAW 264.7) was treated with phorbol myristate acetate (PMA). Additionally, coelenterazine was used to monitor the changes in ROS production over time in a mouse model of IBD.

Results: In vitro, coelenterazine enabled the dynamic monitoring of the RAW 264.7 cell oxidative burst. In vivo, there were early, preclinical, changes in the localization and magnitude of coelenterazine chemiluminescent foci.

Conclusions: Coelenterazine offers a high-throughput method for assessing immune cell activation in culture and provides a means for the in vivo detection and localization of ROS during IBD disease progression.

Keywords: Coelenterazine; Imaging; Inflammatory bowel disease; Reactive oxygen species; Superoxide anion.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Disease Models, Animal
Imaging, Three-Dimensional*
Imidazoles / metabolism
Inflammatory Bowel Diseases / diagnosis*
Inflammatory Bowel Diseases / metabolism*
Luminescent Measurements
Male
Mice
Mice, Inbred BALB C
Pyrazines / metabolism
RAW 264.7 Cells
Reactive Oxygen Species / metabolism*

Substances

Imidazoles
Pyrazines
Reactive Oxygen Species
coelenterazine