Microsatellite instability of gastric cancer and precancerous lesions

Bing Li; Hong-Yi Liu; Shao-Hua Guo; Peng Sun; Fang-Ming Gong; Bao-Qing Jia

Microsatellite instability of gastric cancer and precancerous lesions

Int J Clin Exp Med. 2015 Nov 15;8(11):21138-44. eCollection 2015.

Authors

Bing Li¹, Hong-Yi Liu¹, Shao-Hua Guo¹, Peng Sun¹, Fang-Ming Gong¹, Bao-Qing Jia¹

Affiliation

¹ Department of Surgical Oncology, General Hospital of The People's Liberation Army No. 28, Fuxing Road, Beijing 100853, P.R. China.

PMID: 26885046
PMCID: PMC4723891

Abstract

Objective: To investigate whether microsatellite instability (MSI) of gastric cancer and precancerous lesions were existed and its effect.

Methods: Laser microdissection was used. Gastric, intestinal metaplasia, dysplasia and normal mucosa were collected respectively. Five microsatellite loci were selected and MSI was detected by denaturing high-performance liquid chromatography.

Results: In the five microsatellite loci REF-positive phenotype, intestinal metaplasia MSI was 20.7%. Dysplasia MSI was 22.4%. Gastric MSI was 47.9%, and there was no MSI in normal gastric mucosa.

Conclusion: MSI gradually increased from precancerous lesions to gastric cancer. The early detection of MSI may be a potential early warning indicator for early diagnosis of gastric cancer.

Keywords: Gastric cancer; denaturing high performance liquid chromatography; laser micro dissection; microsatellite instability; precancerous lesions.