Abstract
Herein we report molecular shape-dependent nonspecific labeling of photoaffinity linkers (PLs) in the cellular proteome. Linear PLs have a greater tendency to engage in nonspecific binding than branched PLs. Exploiting this property, we discovered a smaller branched diazirine-based PL as the best photoaffinity probe with minimal nonspecific binding characteristics from among 5 probes with different PLs.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Benzophenones / chemistry*
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Click Chemistry
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Diazomethane / analogs & derivatives*
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Diazomethane / chemistry*
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Electrophoresis, Gel, Two-Dimensional
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HeLa Cells
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Humans
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L-Lactate Dehydrogenase / analysis
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Molecular Structure
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Photoaffinity Labels / chemistry*
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Tubulin / analysis*
Substances
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Benzophenones
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Photoaffinity Labels
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Tubulin
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Diazomethane
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L-Lactate Dehydrogenase