Abstract
A cationic molecular peptide beacon NAP1 functionalized with a fluorescence resonance energy transfer-pair at its ends allows the ratiometric detection of ds-DNA with a preference for AT rich sequences. NAP1 most likely binds in a folded form into the minor groove of ds-DNA, which results in a remarkable change in its fluorescence properties. As NAP1 exhibits quite low cytotoxicity, it can also be used for imaging of nuclear DNA in cells.
MeSH terms
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A549 Cells
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Animals
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Binding Sites
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Cattle
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Cell Survival / drug effects
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DNA / analysis*
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Fluorescence Resonance Energy Transfer*
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Microscopy, Confocal
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Naphthalenes / chemistry*
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Naphthalenes / metabolism
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Naphthalenes / toxicity
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Oligonucleotides / chemistry
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Oligopeptides / chemistry*
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Oligopeptides / metabolism
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Oligopeptides / toxicity
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Peptides / chemistry*
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Peptides / metabolism
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Peptides / toxicity
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Pyrenes / chemistry
Substances
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Naphthalenes
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Oligonucleotides
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Oligopeptides
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Peptides
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Pyrenes
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locked nucleic acid
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nucleic acid probe 1
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DNA
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calf thymus DNA
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pyrene