Objective: To establish the patent ductus arteriosus model in preterm rats using the improved natural development method.
Methods: In the light of the flaws of the natural development method in establishing the patent ductus arteriosus model, the experimental technology was modified to avoid the influence of fixation, dehydration, and section method on blood vessel diameter. Cesarean section was performed for a Wistar rat pregnant for 19 days, and 8 neonatal rats were obtained. After they were sacrificed by dislocation, they were embedded as a whole to avoid dehydration, and the microsection and horizontal section were made. After hematoxylin and eosin staining, a microscope was used to measure the inner diameters of the ductus arteriosus, the main pulmonary artery, and the descending aorta.
Results: After the cesarean section for the rat pregnant for 19 days, patent ductus arteriosus occurred in all the 8 neonatal rats. The measurements of the inner diameters of blood vessels were as follows: the long diameter and short diameter of the descending aorta were 354±106 and 182±140 μm, respectively; the short diameter of the ductus arteriosus was 155±122 μm, and its area was 36,847±42,582 μm(2); the long axis and short axis of the main pulmonary artery were 589±150 and 174±170 μm, respectively.
Conclusions: The improved natural development method can help to successfully establish the patent ductus arteriosus model in preterm rats.
目的: 利用改良的自然发育法构建早产大鼠动脉导管开放模型。
方法: 基于自然发育法在构建早产大鼠动脉导管开放模型上的缺陷, 本研究对实验技术进行了改进, 避免固定方式、脱水处理及切片方式对血管管径的影响。将1只孕19 d的Wistar大鼠行剖宫产, 取出8只新生大鼠, 脱臼处死后整体包埋、避免脱水、微距切片、水平切片, 苏木精-伊红染色后镜下测量动脉导管、主肺动脉、降主动脉内径。
结果: 孕19 d大鼠剖宫产术后8只新生大鼠动脉导管均开放。各血管内径测量结果显示:降主动脉长径:354±106 μm, 短径:182±140 μm; 动脉导管短径:155±122 μm, 面积:36 847±42 582 μm2; 主肺动脉长轴:589±150 μm, 短轴:174±170 μm。
结论: 改良的早产大鼠动脉导管开放模型成功构建。