Emergence of NDM-5- and MCR-1-Producing Escherichia coli Clones ST648 and ST156 from a Single Muscovy Duck (Cairina moschata)

Run-Shi Yang; Youjun Feng; Xiao-Yue Lv; Jia-Hong Duan; Jing Chen; Liang-Xing Fang; Jing Xia; Xiao-Ping Liao; Jian Sun; Ya-Hong Liu

doi:10.1128/AAC.01365-16

Emergence of NDM-5- and MCR-1-Producing Escherichia coli Clones ST648 and ST156 from a Single Muscovy Duck (Cairina moschata)

Antimicrob Agents Chemother. 2016 Oct 21;60(11):6899-6902. doi: 10.1128/AAC.01365-16. Print 2016 Nov.

Authors

Run-Shi Yang^{1

2}, Youjun Feng³, Xiao-Yue Lv^{1

2}, Jia-Hong Duan^{1

2}, Jing Chen^{1

2}, Liang-Xing Fang^{1

2}, Jing Xia^{1

2}, Xiao-Ping Liao^{1

2}, Jian Sun^{4

2}, Ya-Hong Liu^{4

2}

Affiliations

¹ National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, South China Agricultural University, Guangzhou, People's Republic of China.
² Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, South China Agricultural University, Guangzhou, People's Republic of China.
³ Department of Medical Microbiology and Parasitology, Zhejiang University School of Medicine, Zhejiang, People's Republic of China.
⁴ National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, South China Agricultural University, Guangzhou, People's Republic of China jiansun@scau.edu.cn lyh@scau.edu.cn.

Abstract

Two Escherichia coli clones (sequence type 648 [ST648] and ST156) that coproduce NDM-5 and MCR-1 were detected from a single fowl in China. The bla_NDM-5 gene was found on the two indistinguishable IncX3 plasmids from the two different E. coli isolates, whereas the mcr-1 gene was located on IncHI2 and IncI2 plasmids, respectively, suggesting that bla_NDM-5 and mcr-1 have spread in avian intestinal flora. Also, the two strains harbor bla_TEM-1, bla_CTX-M-55, fosA3, and aac(6')-Ib The multiresistant E. coli strains (especially the epidemic clone ST648) might raise a potential threat to human health via food chain transmission.

MeSH terms

Animals
China
Drug Resistance, Multiple, Bacterial / drug effects*
Drug Resistance, Multiple, Bacterial / genetics
Ducks / microbiology
Escherichia coli / drug effects*
Escherichia coli / genetics*
Escherichia coli / isolation & purification
Escherichia coli Proteins / genetics*
Microbial Sensitivity Tests
beta-Lactamases / genetics*

Substances

Escherichia coli Proteins
MCR-1 protein, E coli
beta lactamase NDM-5, E coli
beta-Lactamases