Alcohol exposure impairs trophoblast survival and alters subtype-specific gene expression in vitro

J I Kalisch-Smith; J E Outhwaite; D G Simmons; M Pantaleon; K M Moritz

doi:10.1016/j.placenta.2016.08.080

Alcohol exposure impairs trophoblast survival and alters subtype-specific gene expression in vitro

Placenta. 2016 Oct:46:87-91. doi: 10.1016/j.placenta.2016.08.080. Epub 2016 Aug 25.

Authors

J I Kalisch-Smith¹, J E Outhwaite¹, D G Simmons¹, M Pantaleon¹, K M Moritz²

Affiliations

¹ School of Biomedical Sciences, University of Queensland, QLD, 4072, Australia.
² School of Biomedical Sciences, University of Queensland, QLD, 4072, Australia. Electronic address: k.moritz1@uq.edu.au.

PMID: 27697226
DOI: 10.1016/j.placenta.2016.08.080

Abstract

Maternal alcohol consumption is common prior to pregnancy recognition and in the rat results in altered placental development and fetal growth restriction. To assess the effect of ethanol (EtOH) exposure on the differentiation of trophoblast stem (TS) cells, mouse TS lines were differentiated in vitro for 6 days in 0%, 0.2% or 1% EtOH. This reduced both trophoblast survival and expression of labyrinth and junctional zone trophoblast subtype-specific genes. This suggests that fetal growth restriction and altered placental development associated with maternal alcohol consumption in the periconceptional period could be mediated in part by direct effects on trophoblast development.

Keywords: Apoptosis; Differentiation; Ethanol; Placenta; Programming.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Biomarkers / metabolism
Cell Differentiation / drug effects
Cell Proliferation / drug effects
Central Nervous System Depressants / toxicity*
Ethanol / toxicity*
Gene Expression / drug effects
Mice
Pregnancy Proteins / metabolism*
Trophoblasts / drug effects*
Trophoblasts / metabolism

Substances

Biomarkers
Central Nervous System Depressants
Pregnancy Proteins
Ethanol