Protein-based nanotoxicology assessment strategy

Marlene Pedersen Elnegaard; Markus List; Helle Christiansen; Steffen Schmidt; Jan Mollenhauer; Ines Block

doi:10.1016/j.nano.2016.12.020

Protein-based nanotoxicology assessment strategy

Nanomedicine. 2017 Apr;13(3):1229-1233. doi: 10.1016/j.nano.2016.12.020. Epub 2017 Jan 5.

Authors

Marlene Pedersen Elnegaard¹, Markus List², Helle Christiansen³, Steffen Schmidt³, Jan Mollenhauer³, Ines Block³

Affiliations

¹ Lundbeckfonden Center of Excellence in Nanomedicine NanoCAN, University of Southern Denmark, Odense, Denmark; Institute of Molecular Medicine, University of Southern Denmark, Odense, Denmark. Electronic address: mlpedersen@health.sdu.dk.
² Lundbeckfonden Center of Excellence in Nanomedicine NanoCAN, University of Southern Denmark, Odense, Denmark; Institute of Molecular Medicine, University of Southern Denmark, Odense, Denmark; Institute of Clinical Research, University of Southern Denmark, Odense, Denmark.
³ Lundbeckfonden Center of Excellence in Nanomedicine NanoCAN, University of Southern Denmark, Odense, Denmark; Institute of Molecular Medicine, University of Southern Denmark, Odense, Denmark.

PMID: 28064007
DOI: 10.1016/j.nano.2016.12.020

Abstract

The nanomaterial community calls for standardized in vitro assays to determine nanoparticle toxicity in the effort to reduce the number of in vivo validation experiments. We demonstrate that chip-based protein detection is suitable for assessing toxicity and may complement traditional assays to improve selection of primary hits for subsequent analysis. As nanodrug mimics, we analyzed the effect of transiently transfected siRNAs in MCF7 breast cancer cells and normal MCF12A breast cells, resembling a differential screen. As a measure of cytotoxicity, we determined cell viability as well as protein expression of glyceraldehyde-3-phosphate dehydrogenase, transferrin receptor, and the proliferation marker Ki67. The evaluation of cell lethality and protein expression unraveled cellular effects overseen by one method alone.

Keywords: Cell viability; Nanotoxicology; Protein array; RNAi.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Breast / cytology
Cell Line
Cell Line, Tumor
Cell Survival / drug effects
Female
Gene Expression Regulation / drug effects
Glyceraldehyde-3-Phosphate Dehydrogenases / genetics
Humans
Ki-67 Antigen / genetics
Nanoparticles / toxicity*
Protein Array Analysis / methods*
RNA, Small Interfering / genetics
Receptors, Transferrin / genetics
Toxicity Tests / methods*
Transfection

Substances

Ki-67 Antigen
RNA, Small Interfering
Receptors, Transferrin
Glyceraldehyde-3-Phosphate Dehydrogenases