In vitro protective effects of botryosphaeran, a (1→3;1→6)-β-d-glucan, against mutagens in normal and tumor rodent cells

Mutat Res Genet Toxicol Environ Mutagen. 2017 Feb:814:29-36. doi: 10.1016/j.mrgentox.2016.12.003. Epub 2016 Dec 31.

Abstract

Botryosphaeran (BOT) is an exocellular β-d-glucan (carbohydrate biopolymer) of the (1→3;1→6)-linked type produced by Botryosphaeria rhodina MAMB-05. The cytotoxic, mutagenic, genotoxic, and protective effects of this substance were evaluated in Chinese hamster lung fibroblasts (V79) and rat hepatocarcinoma cells (HTC) by the micronucleus test (MN) and the comet assay. BOT was not genotoxic in either cell line; it decreased the clastogenic effects of doxorubicin, H2O2, and benzo[a]pyrene. These results indicate that BOT may have potential as a therapeutic agent.

Keywords: Benzo[a]pyrene; Chemoprevention; Doxorubicin; HTC cells; Hydrogen peroxide; V79 cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antimutagenic Agents / pharmacology*
  • Cricetinae
  • Cricetulus
  • Glucans / pharmacology*
  • In Vitro Techniques
  • Mutagenicity Tests
  • Rats

Substances

  • Antimutagenic Agents
  • Glucans
  • botryosphaeran