Characterization of pHV2 from Halobacterium volcanii and its use in demonstrating transformation of an archaebacterium

Proc Natl Acad Sci U S A. 1987 Dec;84(23):8530-4. doi: 10.1073/pnas.84.23.8530.

Abstract

We determined the complete nucleotide sequence of the 6354-base-pair plasmid pHV2 of the archaebacterium Halobacterium volcanii. This plasmid is present in approximately six copies per chromosome. We have generated a strain, H. volcanii WFD11, cured of pHV2 by treatment of liquid cultures with ethidium bromide. We describe PEG-mediated transformation of H. volcanii WFD11 with intact pHV2 and with a form of pHV2 marked by a 93-base-pair deletion generated in vitro.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Chromosome Mapping
  • DNA Restriction Enzymes
  • DNA, Bacterial / genetics
  • Genes, Bacterial
  • Genetic Vectors*
  • Halobacterium / genetics*
  • Molecular Sequence Data
  • Plasmids*
  • Transformation, Genetic

Substances

  • DNA, Bacterial
  • DNA Restriction Enzymes

Associated data

  • GENBANK/J03014