Lipid quantitation and metabolomics data from vitamin E-deficient and -sufficient zebrafish embryos from 0 to 120 hours-post-fertilization

Melissa McDougall; Jaewoo Choi; Hye-Kyeong Kim; Gerd Bobe; J Frederik Stevens; Enrique Cadenas; Robert Tanguay; Maret G Traber

doi:10.1016/j.dib.2017.02.046

Lipid quantitation and metabolomics data from vitamin E-deficient and -sufficient zebrafish embryos from 0 to 120 hours-post-fertilization

Data Brief. 2017 Feb 21:11:432-441. doi: 10.1016/j.dib.2017.02.046. eCollection 2017 Apr.

Authors

Melissa McDougall¹, Jaewoo Choi², Hye-Kyeong Kim³, Gerd Bobe², J Frederik Stevens⁴, Enrique Cadenas⁵, Robert Tanguay⁶, Maret G Traber⁷

Affiliations

¹ Linus Pauling Institute, Oregon State University, Corvallis, OR 97331, USA; College of Public Health and Human Sciences, Oregon State University, Corvallis, OR 97331, USA.
² Linus Pauling Institute, Oregon State University, Corvallis, OR 97331, USA.
³ The Catholic University of Korea, Seoul, Republic of Korea.
⁴ Linus Pauling Institute, Oregon State University, Corvallis, OR 97331, USA; College of Pharmacy, Oregon State University, Corvallis, OR 97331, USA; Environmental Health Sciences Center, Oregon State University, Corvallis, OR 97331, USA.
⁵ University of Southern California, School of Pharmacy, Los Angeles, CA 90089, USA.
⁶ Environmental Health Sciences Center, Oregon State University, Corvallis, OR 97331, USA; Environmental and Molecular Toxicology, Oregon State University, Corvallis, OR 97331, USA; Sinnhuber Aquatic Research Laboratory, Oregon State University, Corvallis, OR 97331, USA.
⁷ Linus Pauling Institute, Oregon State University, Corvallis, OR 97331, USA; College of Public Health and Human Sciences, Oregon State University, Corvallis, OR 97331, USA; Environmental Health Sciences Center, Oregon State University, Corvallis, OR 97331, USA.

Abstract

The data herein is in support of our research article by McDougall et al. (2017) [1], in which we used our zebrafish model of embryonic vitamin E (VitE) deficiency to study the consequences of VitE deficiency during development. Adult 5D wild-type zebrafish (Danio rerio), fed defined diets without (E-) or with VitE (E+, 500 mg RRR-α-tocopheryl acetate/kg diet), were spawned to obtain E- and E+ embryos that we evaluated using metabolomics and specific lipid analyses (each measure at 24, 48, 72, 120 hours-post-fertilization, hpf), neurobehavioral development (locomotor responses at 96 hpf), and rescue strategies. Rescues were attempted using micro-injection into the yolksac using VitE (as a phospholipid emulsion containing d₆-α-tocopherol at 0 hpf) or D-glucose (in saline at 24 hpf).

Keywords: Alpha-tocopherol; Choline; Docosahexaenoic acid; Ferroptosis; Glucose.

Abstract

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