Anomalous binding of DPDPE as a result of batch variability

NIDA Res Monogr. 1986:75:201-4.

Abstract

The binding of the delta selective agonist [3H]DPDPE to diencephalic rat brain tissue was examined. Although most material examined gave rise to a single receptor model, one commercial preparation (Amersham, batch 8) suggested a two receptor model. Purification by HPLC removed a minor component and restored a single receptor model to the major component.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Brain / metabolism*
  • Enkephalin, D-Penicillamine (2,5)-
  • Enkephalins / isolation & purification
  • Enkephalins / metabolism*
  • Enkephalins / standards
  • Quality Control
  • Rats
  • Receptors, Opioid / metabolism*
  • Receptors, Opioid, delta

Substances

  • Enkephalins
  • Receptors, Opioid
  • Receptors, Opioid, delta
  • Enkephalin, D-Penicillamine (2,5)-