Multi-center evaluation of one commercial and 12 in-house real-time PCR assays for detection of Mycoplasma pneumoniae

Diagn Microbiol Infect Dis. 2017 Jun;88(2):111-114. doi: 10.1016/j.diagmicrobio.2017.03.004. Epub 2017 Mar 8.

Abstract

Detection of Mycoplasma pneumoniae by real-time PCR is not yet standardized across laboratories. We have implemented a standardization protocol to compare the performance of thirteen commercial and in-house approaches. Despite differences on threshold values of samples, all assays were able to detect at least 20M. pneumoniae genomes per reaction.

Keywords: Detection; Multi-center evaluation; Mycoplasma pneumoniae; Real-time PCR.

Publication types

  • Multicenter Study

MeSH terms

  • DNA, Bacterial / analysis*
  • DNA, Bacterial / genetics
  • Genome, Bacterial / genetics*
  • Humans
  • Mycoplasma pneumoniae / genetics*
  • Mycoplasma pneumoniae / isolation & purification
  • Pneumonia, Mycoplasma / diagnosis*
  • Pneumonia, Mycoplasma / microbiology
  • Real-Time Polymerase Chain Reaction / methods*

Substances

  • DNA, Bacterial