The establishment of appropriate methods for egg-activation by human PLCZ1 RNA injection into human oocyte

Abstract

Phospholipase C-zeta (PLCZ1), a strong candidate of egg-activating sperm factor, can induce Ca²⁺ oscillations and cause egg activation. For the application of PLCZ1 to clinical use, we examined the pattern of Ca²⁺ responses and developmental rate by comparing PLCZ1 RNA injection methods with the other current methods, such as cytosolic aspiration, electrical stimulation and ionomycin treatment in human oocytes. We found that the pattern of Ca²⁺ oscillations after PLCZ1 RNA injection exhibited similar characteristics to that after ICSI treatment. We also determined the optimal concentration of human PLCZ1 RNA to activate the human oocytes. Our findings suggest that human PLCZ1 RNA is a better therapeutic agent to rescue human oocytes from failed activation, leading to normal and efficient development.

Keywords: Ca(2+) oscillations; Egg activation; Human oocyte; PLCZ1 RNA; Preimplantation development..