New real time and conventional RT-PCRs for updated molecular diagnosis of infectious bronchitis virus infection (IBV) in chickens in Egypt associated with frequent co-infections with avian influenza and Newcastle Disease viruses

J Virol Methods. 2017 Jul:245:19-27. doi: 10.1016/j.jviromet.2017.02.018. Epub 2017 Mar 21.

Abstract

In Egypt, currently two geographically restricted genotypes of the infectious bronchitis coronavirus (IBV) are circulating with detrimental effects for poultry industry. A sensitive real-time RT-PCR assay targeting the IBV nucleocapsid gene (N) was developed to screen clinical samples for presence of IBV. Conventional RT-PCRs amplifying hypervariable regions (HVRs 1-2 and 3) of the IBV S1 gene were developed and amplificates used for nucleotide sequence-based typing of IBV field strains in Egyptian chickens directly from clinical samples.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chickens / virology*
  • Coinfection / diagnosis
  • Coinfection / veterinary*
  • Coinfection / virology
  • Coronavirus Infections / complications
  • Coronavirus Infections / diagnosis
  • Coronavirus Infections / veterinary*
  • Coronavirus Infections / virology
  • Egypt
  • Genotype
  • Infectious bronchitis virus / genetics
  • Infectious bronchitis virus / isolation & purification*
  • Influenza A virus / genetics
  • Influenza in Birds / complications
  • Influenza in Birds / virology
  • Molecular Diagnostic Techniques
  • Newcastle Disease / complications
  • Newcastle Disease / virology
  • Newcastle disease virus / genetics
  • Poultry Diseases / diagnosis*
  • Poultry Diseases / epidemiology
  • Poultry Diseases / virology
  • RNA, Viral / genetics
  • Real-Time Polymerase Chain Reaction / methods*
  • Reverse Transcriptase Polymerase Chain Reaction / methods*

Substances

  • RNA, Viral