Pico-HPLC system integrating an equal inner diameter femtopipette into a 900 nm I.D. porous layer open tubular column

Ruonan Li; Yunlong Shao; Yanmin Yu; Xiayan Wang; Guangsheng Guo

doi:10.1039/c7cc00799j

Pico-HPLC system integrating an equal inner diameter femtopipette into a 900 nm I.D. porous layer open tubular column

Chem Commun (Camb). 2017 Apr 6;53(29):4104-4107. doi: 10.1039/c7cc00799j.

Authors

Ruonan Li¹, Yunlong Shao¹, Yanmin Yu¹, Xiayan Wang¹, Guangsheng Guo¹

Affiliation

¹ Beijing Key Laboratory for Green Catalysis and Separation, Department of Chemistry and Chemistry Engineering, Beijing University of Technology, Beijing 100124, China. xiayanwang@bjut.edu.cn.

PMID: 28349149
DOI: 10.1039/c7cc00799j

Abstract

A picoflow high performance liquid chromatography (pico-HPLC) system was developed, which could directly pipette femtoliter samples using a separation column tip driven by an electroosmotic pump. Amino acid enantiomers were separated in the 900 nm I.D. porous layer open tubular column at a flow rate of 13.50 pL min^-1.